Isolation of Cytotoxic Natural Products from Botanical Sources
The primary research project in the Still Lab is the use of diverse sources of botanical material, collaborative cytotoxicity screening and NMR-based organic structure analysis to discover bioactive chemistry from plants. The two core areas of this project include cytotoxicity screening and NMR-based organic structure analysis. The biological screening component involves a sulforhodamine B assay performed in the Still Lab at CSUDH and an expanded 19-human tumor cell line panel of Alamar Blue-based assays at local biotechnology company, Molecular Express, Inc., located 5 miles from CSUDH. This research project facilitates discovery of natural products from plants by involving undergraduate student researchers in both the Minority Biomedical Research Support (MBRS) and the Louis Stokes Alliances for Minority Participation (LSAMP) programs on the CSUDH campus.
ORCID, Patrick Still: orcid.org/0000-0002-6773-7731
Selected publications in this area:
1. David M. Lucas, Patrick C. Still, Lynette Bueno Pérez, Michael R. Grever, and A. Douglas Kinghorn (2010). Potential of Plant-Derived Natural Products in the Treatment of Leukemia and Lymphoma. Current Drug Targets 11: 812-822. (Invited review article)
2. Yulin Ren, Min Wei, Patrick C. Still, Shunzong Yuan, Youcai Deng, Xiaozhuo Chen, Klaus Himmeldirk, A. Douglas Kinghorn, and Jianhua Yu (2012). Synthesis and Antitumor Activity of Ellagic Acid Peracetate. ACS Medicinal Chemistry Letters 3: 631-636.
3. Patrick C. Still, Bitna Yi, Ryan Pavlovicz, Tatiana F. González-Cestari, Li Pan, Hee-Byung Chai, Tran Hgoc Ninh, Djaja D. Soejarto, Chenglong Li, James R. Fuchs, Dennis McKay, and A. Douglas Kinghorn (2013). Alkaloids from Microcos paniculata L. With Cytotoxic and Nicotinic Receptor Antagonistic Activities. Journal of Natural Products 76: 243-249.
4. Lynette Bueno Pérez, Patrick C. Still, C. Benjamin Naman, Yulin Ren, Li Pan, Hee-Byung Chai, Esperanza J. Carcache de Blanco, Tran Ngoc Ninh, Bui Van Thanh, Steven M. Swanson, Djaja D. Soejarto, and A. Douglas Kinghorn (2014). Investigation of Vietnamese Plants for Potential Anticancer Agents. Phytochemistry Reviews 1-13.
5. Patrick C. Still, Tyler A. Johnson, and Phillip Crews (2014). Scrutinizing the Scaffolds of Marine Biosynthetics from Different Source Organisms: Gram-Negative Cultured Bacterial Products Enter Center Stage. Journal of Natural Products 77: 690-702. (Invited review article)
6. Christine M. Theodore, Nicholas Lorig-Roach, Patrick C. Still, Tyler A. Johnson, Joshua A. Schwochert, Marija Drašković, Cassandra N. Naphen, Mitchell S. Crews, Simone A. Barker, Steven L. Loveridge, Frederick A. Valeriote , Walter M. Bray, R. Scott Lokey, Hsiau W. Lee, and Phillip Crews. (2015). Biosynthetic Products from a Nearshore-Derived Gram-Negative Bacterium Enable Reassessment of the Kailuin Depsipeptides. Journal of Natural Products 78: 441-452.
7. Nicholas Lorig-Roach, Patrick C. Still, David Coppice, Jennifer E. Compton, Mitchell S. Crews, Gabriel Navarro, Karen Tenney, and Phillip Crews. (2017) Evaluating Nitrogen-Containing Biosynthetic Products Produced by Saltwater Culturing of Several California Littoral Zone Gram-Negative Bacteria. Journal of Natural Products 80: 2304-2310.